Published in the Journal of Agricultural and Food Chemistry, a joint study between the Roswell Park Cancer Institute, New Zealand Institute for Crop and Food Research Limited and the Johns Hopkins University, found that bladder tissue is particularly responsive to Sulforaphane. Rats were fed a broccoli sprout extract via oral intubation or included in their dietary feed.  Subsequent urinary output showed dose dependent high levels of isothiocyanate metabolites with no sign of toxicity.  These findings suggest that Sulforaphane may be especially effective in protecting against bladder cancer. (Zhang, Y, et al., November 2006).